Prostate cancer (CaP) is most commonly diagnosed non-dermatological solid malignancy with a high metastatic rate. The growing prevalence of cancer survivors was estimated to be over 28 million worldwide. CaP is the second most common cancer in males of USA and high incidence has been reported in United Kingdom, African, Brazilian and Pakistani men. p53 has an important role in CaP. It plays an critical role in cancer metastasis. The tumor suppressor gene, p53, was found to be at the center of hub of significant biological pathways Reactive oxygen species (ROS) could activate some specific signaling pathways that contribute to tumor development by regulating cell proliferation, angiogenesis and metastasis processes. Current study was designed to determine expression of p53 in CaP as compared to normal control. This is a Cross-sectional analytical study which was conducted at Department of Biochemistry & Molecular Biology & Department of Urology, Rawalpindi, National University of Science and Technology, Islamabad over a period of one year. Study included 32 samples. Expression of p53 was determined by real time qPCR. It has been seen that CaP is an age related disease. Optimum ann ukryj opis