Preface...

Table of Contents...

Contributing Authors...

1. Protein Stains and Applications

Pazhani Sundaram

1. The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel

J.P. Dean Goldring

1. Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE

Gaurav Kumar

1. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann

1. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue

Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang

1. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue

Biji T. Kurien and R. Hal Scofield

1. Silver Staining Techniques of Polyacrylamide Gels

Nicole Berndt, Rolf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann

1. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

Sun-Yong Hwang and Jung-Kap Choi

1. Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain

Sun-Yong Hwang, Xu Wang, and Jung-Kap Choi

1. Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins

Jennie R. Lill and Victor J. Nesatyy

1. Fluorescent Staining of Gels

Engelbert Buxbaum

1. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis

Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni

1. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE

Na Na and Jin Ouyang

1. Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain

Padmaja Mehta-D'souza

1. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain

Biji T. Kurien, Yaser Dorri, and R. Hal Scofield

1. Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis

Divakar, K., Deepa Arul Priya, J, Panneer Selvam, G., Suryia Prabha, M., Ashwin Kannan, Nandhini Devi, G., and Pennathur Gautam

1. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis

Han-Min Chen

1. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

Jelena Bogdanovic Pristov and Ivan Spasojevic

1. Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black

J.P. Dean Goldring and Robert G.E. Krause

1. In-Gel Protein Phosphatase Assay using Fluorogenic Substrates

Isamu Kameshita, Noriyuki Sueyoshi, and atsuhiko Ishida

1. Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride

Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi

1. Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan

Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards

1. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel

Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend

1. Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins

Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, and Paola Pietrangeli

1. A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH

Gaurav Kumar

1. Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

Gaurav