Preface.- 1 General mechanisms of gene vaccines.- 2 Strategies to Improve DNA Vaccine Potency: HPV-associated Cervical Cancer as a Model System.- 3 Immune-activating mechanisms of replicase-based DNA and RNA vaccines and their role in immune-apoptosis.- 4 Cytokine genes as molecular adjuvants for DNA vaccines.- 5 Pharmaceutical non-viral formulations for gene vaccines.- 6 Rational design of formulated DNA vaccines: the DermaVir approach.- 7 In vivo electroporation.- 8 Electroporation-based Trigrid(TM) delivery system (TDS) for DNA vaccine administration.- 9 Prime Boost Regimens for Enhancing Immunity: Magnitude, Quality of Mucosal and Systemic Gene Vaccines.- 10 Intranodal genetic immunization.- 11 Immunotherapy of cancer with RNA vaccines.- 12 DNA and RNA vaccines for prophylactic and therapeutic treatment of type I allergy.- 13 Approved veterinary vaccines - paving the way to products for human use.- 14 Current status of regulations for DNA vaccines.- 15 State of the art production of antibiotic resistance-free plasmid DNA.- 16 Industrial Manufacturing of Plasmid-DNA Products for Gene Vaccination and Therapy.