Series Preface... Preface... Table of Contents... Contributing Authors... Part I Introduction 1. Brain Derived Neurotrophic Factor and the Attività plastic dei neuroni, the Neuronal Plasticity as Defined by Ernesto Lugaro (1870-1940) Heather Bowling and Moses V. Chao Part II Transcripts of BDNF 2. Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes Kaur Jaanson, Angela Pärn, and Tõnis Timmusk 3. Detecting Single and Multiple BDNF Transcripts In Situ in Neuronal Cultures and Brain Sections Andrea Colliva, Kristen R. Maynard, Keri Martinowich, and Enrico Tongiorgi 4. Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons Chinnavuth Vatanashevanopakorn, Amir Grover, Arup R. Nath, Kevin Clark, Paul Sopp, Claus Nerlov, and Liliana Minichiello 5. Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from rare of Limited Samples of Adult and Aged Central Neurons Arup R. Nath, Roy Drissen, Fei Guo, Claus Nerlov, and Liliana Minichiello Part III Protein Forms of BDNF 6. Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence Stefano Donegà and Enrico Tongiorgi 7. Methodology for Detecting and Tracking brain-Derived Neurotrophic Factor Complexes in Neurons using Single Quantum Dots Anke Vermehren-Schmaedick, Thomas Jacob, and Tania Q. Vu 8. Recording Activity-Dependent Release of BDNF from Hippocampal Neurons Tanja Brigadski, Petra Lichtenecker, and Volkmar Lessmann Part IV Receptors of BDNF 9. Ultrastructural Localization of BDNF and trkB Receptors Chiara Salio and Adalberto Merighi 10. Analysis of TrkB Receptor Activity using FRET Sensors Charles E. hall, James O. McNamara, and Ryohei Yasuda Part V Signaling Cascades of BDNF 11. BDNF-Induced Intracellular Signaling João R. Gomes, Andrea Lobo, Carlos B. Duarte, and Mário Grãos 12. A Microfluidic Culture Platform for Neurotrophin Signaling Studies Rui O. Costa, Tânia Perestrelo, Diogo Tomé, and Ramiro D. Almeida Part VI BDNF-Induced Protein Synthesis and Synaptic Regulation 13. BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-azidohomoalanine Victor Briz and Michel Baudry 14. Proteomic Tools to Study the Effect of BDNF on de novo Protein Synthesis Heather Bowling and Eric Klann 15. BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus in Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation Debabrata Panja and Clive Bramham ukryj opis