Preface...

Table of Contents...

Contributing Authors...

1. Tools in the Art of Studying Necroptosis

Adrian T. Ting

1. Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules

David Mark Moquin and Francis Ka-Ming Chan

1. Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis

Danish Saleh and Alexei Degterev

1. Distinguishing Necroptosis from Apoptosis

Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, and Motti Gerlic

1. Methods for Studying TNF-Mediated Necroptosis in Cultured Cells

Zikou Liu, John Silke, and Joanne M. Hildebrand

1. Analysis of Necroptosis in Bone Marrow-Derived Macrophages

Diana Legarda and Adrian T. Ting 7. Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis

Diego A. Rodriguez and Douglas R. Green

1. Detection of MLKL Oligomerization during Programmed Necrosis

Zhenyu Cai and Zheng-Gang Liu

1. Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation

Roshan J. Thapa, Shoko Nogusa, and Siddharth Balachandran

1. Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis

Rosalind L. Ang and Adrian T. Ting

1. Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis

Allison M. Beal, John Bertin, and Michael A. Reilly

1. Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock

Matija Zelic and Michelle A. Kelliher

1. Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury

Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, and Andreas Linkermann

1. Assessment of In Vivo Kidney Cell Death: Glomerular Injury

Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, and Andreas Linkermann

1. Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling

Joshua D. Webster, Margaret Solon, Susan Haller, and Kim Newton 16. Characterization of the TNFR1-SC using 'Modified Tandem Affinity Purification' in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS)

Matthias Reichert, Amandeep Bhamra, Sebastian Kupka, and Henning Walczak

1. Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex

Dario Priem, Yves Dondelinger, and Mathieu JM Bertrand 18. Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages

Diana Legarda and Adrian T. Ting