Preface... Table of Contents... Contributing Authors... 1. Tools in the Art of Studying Necroptosis Adrian T. Ting 2. Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules David Mark Moquin and Francis Ka-Ming Chan 3. Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis Danish Saleh and Alexei Degterev 4. Distinguishing Necroptosis from Apoptosis Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, and Motti Gerlic 5. Methods for Studying TNF-Mediated Necroptosis in Cultured Cells Zikou Liu, John Silke, and Joanne M. Hildebrand 6. Analysis of Necroptosis in Bone Marrow-Derived Macrophages Diana Legarda and Adrian T. Ting 7. Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis Diego A. Rodriguez and Douglas R. Green 8. Detection of MLKL Oligomerization during Programmed Necrosis Zhenyu Cai and Zheng-Gang Liu 9. Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation Roshan J. Thapa, Shoko Nogusa, and Siddharth Balachandran 10. Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis Rosalind L. Ang and Adrian T. Ting 11. Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis Allison M. Beal, John Bertin, and Michael A. Reilly 12. Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock Matija Zelic and Michelle A. Kelliher 13. Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, and Andreas Linkermann 14. Assessment of In Vivo Kidney Cell Death: Glomerular Injury Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, and Andreas Linkermann 15. Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling Joshua D. Webster, Margaret Solon, Susan Haller, and Kim Newton 16. Characterization of the TNFR1-SC using 'Modified Tandem Affinity Purification' in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS) Matthias Reichert, Amandeep Bhamra, Sebastian Kupka, and Henning Walczak 17. Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex Dario Priem, Yves Dondelinger, and Mathieu JM Bertrand 18. Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages Diana Legarda and Adrian T. Ting ukryj opis