1. Microinjection and Micromanipulation: A Historical Perspective

   Wenhao Xu

2. Production of Transgenic Mice by Pronuclear Microinjection

   Xin-an Pu, Anthony P. Young, and H. Michael Kubisch

3. Transgene Recombineering in Bacterial Artificial Chromosomes

   Michael G. Zeidler and Thomas L. Saunders

4. Using TARGATT TM Technology to Generate Site-Specific Transgenic Mice

   Ruby Yanru Chen-Tsai

5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method

   Takuya Sato and Takehiko Ogawa

6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer

   Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu

7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection

   Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong

8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice

   Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney

9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique

   Melissa A. Scott and Yueh-Chiang Hu

10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation

Wenning Qin and Haoyi Wang

1. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides

Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu

1. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection

Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama

1. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications

Naomi Nakagata, Jorge Sztein, and Toru Takeo

1. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm

Toru Takeo, Jorge Sztein, and Naomi Nakagata

1. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line

Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo

1. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology

Tuija M. Alcantar and Xin Y. Rairdan

1. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos

Xiaoxia Cui

1. Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells

Masumi Hirabayashi and Shinichi Hochi

1. Generation of Rabbit Models by Gene Editing Nucleases

Dongshan Yang, Jie Xu, and Y. Eugene Chen

1. Production of Genetically Engineered Porcine Embryos by Handmade Cloning

Rong Li, Jinxin Miao, and Zhongde Wang

1. Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production Qingran Kong and Zhonghua Liu

2. Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer

Zhiqiang Fan, Min Yang, Misha Regouski, and Irina A. Polejaeva

1. Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting

Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, and Fuliang Du

1. Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes Ellen J. Collarini, Philip A. Leighton, and Marie-Cecile van de Lavoir

2. Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing

Jyoti Iyer, Nicole DeVaul, Tyler Hansen, and Ben Nebenfuehr

1. Microinjection in Zebrafish for Genome Editing and Functional Studies

Wuhong Pei and Shawn M. Burgess

1. Microinjection of Marine Fish Eggs

Rie Goto, Taiju Saito, Takahiro Matsubara, and Etsuro Yamaha

1. Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method

Daji Luo, Ke Feng, Zuoyan Zhu, and Wei Hu

1. Functional Studies of Transcriptional Co