1. Microinjection and Micromanipulation: A Historical Perspective Wenhao Xu 2. Production of Transgenic Mice by Pronuclear Microinjection Xin-an Pu, Anthony P. Young, and H. Michael Kubisch 3. Transgene Recombineering in Bacterial Artificial Chromosomes Michael G. Zeidler and Thomas L. Saunders 4. Using TARGATT TM Technology to Generate Site-Specific Transgenic Mice Ruby Yanru Chen-Tsai 5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method Takuya Sato and Takehiko Ogawa 6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu 7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong 8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney 9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique Melissa A. Scott and Yueh-Chiang Hu 10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation Wenning Qin and Haoyi Wang 11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu 12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama 13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications Naomi Nakagata, Jorge Sztein, and Toru Takeo 14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm Toru Takeo, Jorge Sztein, and Naomi Nakagata 15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo 16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology Tuija M. Alcantar and Xin Y. Rairdan 17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos Xiaoxia Cui 18. Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells Masumi Hirabayashi and Shinichi Hochi 19. Generation of Rabbit Models by Gene Editing Nucleases Dongshan Yang, Jie Xu, and Y. Eugene Chen 20. Production of Genetically Engineered Porcine Embryos by Handmade Cloning Rong Li, Jinxin Miao, and Zhongde Wang 21. Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production Qingran Kong and Zhonghua Liu 22. Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer Zhiqiang Fan, Min Yang, Misha Regouski, and Irina A. Polejaeva 23. Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, and Fuliang Du 24. Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes Ellen J. Collarini, Philip A. Leighton, and Marie-Cecile van de Lavoir 25. Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing Jyoti Iyer, Nicole DeVaul, Tyler Hansen, and Ben Nebenfuehr 26. Microinjection in Zebrafish for Genome Editing and Functional Studies Wuhong Pei and Shawn M. Burgess 27. Microinjection of Marine Fish Eggs Rie Goto, Taiju Saito, Takahiro Matsubara, and Etsuro Yamaha 28. Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method Daji Luo, Ke Feng, Zuoyan Zhu, and Wei Hu 29. Functional Studies of Transcriptional Co ukryj opis